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OJVRTM
Online Journal
of Veterinary Research©
Volume 13 (1):86-93, 2009
Polymorphism in TNP-2 gene
and its association with seminal parameters of cattle and buffalo bulls
Panigrahi SK, Yadav BR
Livestock Genome Analysis Laboratory, Dairy Cattle
Breeding Division, National Dairy Research Institute, Karnal
- 132 001,
ABSTRACT
Panigrahi SK, Yadav BR, Polymorphism
in TNP-2 gene and its association with seminal parameters of cattle and buffalo
bulls, Online J Vet Res, 13 (1):86-93, 2009. In the process of sperm maturation, major
restructuring of the somatic chromatin takes place in which the histones are first replaced by a group of arginine and lysine-rich proteins called transition
proteins (TPs); these are in turn replaced by protamines. In the present study transition nuclear
protein-2 (TNP-2) gene was analyzed using PCR-SSCP technique to detect
polymorphism in Sahiwal, Murrah
and Karan Fries bulls. Sequence analysis of TNP-2
gene of Murrah buffalo bulls revealed two single
nucleotide polymorphisms (SNPs) at 182 bp and 186 bp position of intronic region. However, the gene sequences of Sahiwal and Karan-Fries bulls
revealed no variation. The effect of this polymorphism was explored on
individual motility, mass activity and maturation of spermatozoa. Analysis of
variance indicates two variants of Murrah buffalo
have non-significant effect on maturation, mass activity and individual
motility of spermatozoa. Effect of breed of animals has significant effect on
mass activity and individual motility, however it has
non-significant effect on sperm maturation.
Key words:
Polymorphism, SSCP, TNP-2 gene, sperm maturation, cattle, buffalo, bulls