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OJVRTM
Online Journal of Veterinary Research©
Volume
11 (3):
5-13, 2007
Trichinella spiralis
larval antigen for diagnosis
in horses
Hassan SE, Abdel-Rahman EH, Ghazy AA
Department of Parasitology
and Animal Diseases,
Veterinary Research Division, National Research Center, Post Box
12622, 33 El-Tahrir Street, Dokki, Giza, Cairo,
Egypt
ABSTRACT
Hassan SE,
Abdel-Rahman EH, Ghazy
AA,
Trichinella spiralis larval antigen
for diagnosis in horses, Online J Vet Res,
11 (3):
5-13, 2007. An affinity
purification process
using CNBr-Sepharose 4B column
chromatography was used to purify Trichinella
spiralis crude larval extract. The
process
resulted in
a fraction (bound) with 6 bands of 25.7, 36.8, 58.8, 77.9, 150.8 and
174.0 KDa compared with 12 bands
associated with the crude larval
extract (16.6- 174.0 KDa) determined by
sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-
PAGE). Only four
bands (25.7, 36.8, 58.8 and 174.0 KDa) of
the
isolated fraction were immunoreactive with
T. spiralis positive horse sera
detected by Western
blot analysis. The bound fraction was successfully utilized in the
diagnosis of
trichinellosis in horses of different ages,
sexes and
breeds. It showed the highest diagnostic potency (86.9%) using Enzyme
Linked Immunosorbent Assay (ELISA)
compared with crude larval
extract (64.0%) and unbound fraction (52.0%). The current research
introduces a
fraction (bound) isolated from T. spiralis
crude
larval extract, which could be used for detection of T.
spiralis antibodies in horses.
Keywords: Trichinella spiralis; Horse; Affinity
purification;
ELISA; Western Blot.