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OJVRTM
Online Journal of Veterinary Research©
Volume 10 (2) : 134 - 145, 2006
Probiotic potential
of Avian Intestinal Lactic Acid Bacteria
SUMMARY
Mojgani N, Torshizi M, Molae M, Probiotic potential
of Avian Intestinal Lactic
Acid Bacteria Online
J Vet Res 10 (2) : 134-145, 2006
A
total of
250 chicken intestinal specimens were screened for the presence of
Lactic acid
bacteria (LAB) by routine cultural, morphological and biochemical
reactions.
Based on the results, 75 isolates were identified as LAB and were
characterized
for their probiotic potential. During
preliminary investigations,
the viability of the isolated LAB strains under different environmental
and
stress conditions was evaluated. Three of the strains which appeared
resistant
to acidic pH values and high bile salt concentrations were selected for
further
detailed studies. Based on the biochemical reactions these strains were
identified as, Lactobacillus
acidophilus
RNL26, Lactobacillus fermentum RNL
44, and
Lactobacillus salivarrius RNL
49. All three strains demonstrated enhanced inhibitory
activity against gram positive species including Clostridium
perfringens, Staphylococcus
aureus
and Listeria monocytogenes.
Only
L.fermentum
RNL 44 was inhibitory towards gram negative bacteria; Salmonella
enteritidis
and S.typhimurium.
The
antibacterial effect exerted by L. acidophilus RNL26 and L.salivarrius
RNL
49 appeared to be due to bacteriocin
or bacteriocin like substance, as the
neutralized and
catalase treated supernatant fluid of both
the
strains retained their antibacterial activity. All
three strains were further characterized
for their cholesterol removal, aggregation, co-aggregation, and cell
surface hydrophobicity under invitro
conditions.
Key
Words:
Lactic Acid Bacteria
(LAB), Invitro, Antimicrobial, Poultry, Probiotic potential.