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OJVRTM
Online Journal of Veterinary Research©
Volume
Functional
characterization of catalytic
site of Glucose-6-phosphate
dehydrogenase gene in Bos
indicus
Ravikumar G, Satyendra PS, Amrita C, Kaur
T, Narang R
.
Molecular Cytogenetics
Lab, Department of Animal Breeding and Genetics, GAVASU,
ABSTRACT
Ravikumar G, Satyendra
PS, Amrita C, Kaur T, Narang
R . Functional
characterization of catalytic site of Glucose-6-phosphate dehydrogenase
gene in Bos indicus,
Online J Vet
Res 11(1) : 20-28, 2007. Glucose-6-phosphate-dehydrogenase
(G6PD) is an ubiquitous enzyme that
determines NADPH levels
during fatty acid biosynthesis and detoxification of organic peroxides
in cells.
The structural gene for G6PD is X linked in mammals and on the basis of
its
expression in many tissues it can be regarded as a typical housekeeping
gene.
In this study the coding sequence of Bos indicus G6PD mRNA having 1548 bp
encoding 515 amino acids is reported. The 11-residue tryptic
peptide structure containing the reactive lysine assigned for the
catalytic
activity in G6PD was found to be strikingly similar in baker’s yeast,
bovine
and human G6PD.
Key words:
Sequence, Bos indicus, Glucose-6-phosphate-dehydrogenase,
bovine