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OJVRTM
Online Journal of Veterinary Research©
Volume
10 (2)
: 146 -
155, 2006
Variability
in the intron-2 of leptin
gene in Reverine buffaloes
Srikala R, Ravikumar G, Narang
R, Satyendra PS, Amrita C, Rajni G
Molecular Cytogenetics Lab, Department of
Animal Breeding and Genetics, GAVASU,
ABSTRACT
Srikala R, Ravikumar
G, Narang R, Satyendra
PS,
Amrita C, Rajni G Variability in the
intron-2 of leptin gene in Reverine
buffaloes, Online J Vet
Res 10 (2) : 146-155,
2006. A study
on leptin gene was conducted to detect the
variability at nucleotide level in reverine
buffaloes. The PCR-RFLP of 423 bp amplicon
of leptin gene with Hph I, Sau 3A I and Alu
I revealed monomorphism at these loci in Mehsana, Surti and Nili-Ravi breeds of buffaloes. However on
sequencing the amplicon in these breeds
variability at nucleotide
positions 43, 47, 51, 70, 71, 76, 89, 94, 99, 150, 194, 213, 267, 315,
335,411
and 415 was identified. The comparison between buffalo sequences and
other
species revealed changes of nucleotides at several positions. The
sequences
were submitted in the NCBI gene bank with accession numbers: DQ497641,
DQ831142
and DQ831143. On phylogenetic analysis of
nucleic
acid sequences all the buffalo leptin
sequences clustered
into a monophyletic group. The bovids
clustered
clearly from Capra hircus
and Ovis aries
cluster.
Key words : Sequence, polymorphism,
Bubalus bubalis, Leptin