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OJVRTM
Online Journal of Veterinary Research©
Volume
12 (1)
:7-13, 2008
Shahbazkia HRa ,
Nazifi S b*
ab
Department9s0 of Biochemistry and Clinical Pathology, School of
Veterinary Medicine, Shiraz University, P.O. Box 1731, Shiraz 71345,
Iran
Shahbazkia HR, Nazifi
S, Determination
of glycated
haemoglobin (HbG)
and its
correlation with plasma glucose in Iranian fat-tailed sheep and lamb, Online
Journal of Veterinary Research 12 (1): 7-13, 2008. Glycated
haemoglobin (HbG)
concentration
is a retrospective measure of mean blood glucose level and is not
affected by
recent stresses, food ingestion or exercise. HbG
has
been determined in various wild and domestic animals such as kestrels,
mankhor, mouflon,
aoudad,
deer, goat, sheep, dog, camel and horse. But there is no information
about HbG in Iranian fat-tailed sheep and
its relation to blood
glucose. The purposes of this
study were to
determine normal value of glycated haemoglobin in adult Iranian fat-tailed sheep
and lambs and
to investigate its relation to plasma glucose. Blood samples were
collected
from jugular veins of 50 clinically healthy adult sheep (25 males and
25
females) and 30 lambs. After separation and washing of red blood cells,
haemolysate was prepared and subjected to
weak cation exchange chromatography for
determination of HbG. Glucose was measured
in plasma samples. Plasma glucose
and HbG in adult fat-tailed sheep were
69.2 ± 3.24
mg/dl and 2.58 ± 0.26 % of total haemoglobin
and
those of lambs were 84.8 ± 9.31 mg/dl and 3.17 ± 0.29 % of total haemoglobin respectively. It was shown that HbG% and plasma glucose correlated together (r =
0.82,
p<0.05). To determine if low HbG
percent in adult
sheep is related to low permeability of erythrocytes to blood glucose, haemolysates of adult sheep and lambs incubated
with
glucose (70 and 85mg/dl final glucose concentration respectively) were
subjected to chromatography in the same conditions. It was shown that HbG was not significantly increased in glycosylated
haemolysates of lambs but was significantly
increased
in adult sheep. It was concluded that in adult sheep apart from lower
blood
glucose, lower permeability of erythrocytes to blood glucose might be
reason
for lower HbG in comparison with lambs and
some other
species.
KEY WORDS: Glycated
haemoglobin(HbG), Cation
exchange chromatography, Iranian fat-tailed sheep, Lamb, Plasma
glucose, Erythrocyte
permeability.
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