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OJVRTM
Online Journal of Veterinary Research©
Volume 10 (2) : 219-234, 2006
Evaluation of different Brucella
diagnostic techniques on Baladi Does
experimentally
infected
with Brucella melitensis
Abd El-Razik KA, Desouky
HM, Ahmed WM
Department
of
Animal Reproduction & A.I., National Research Center, Dokki, Giza, Egypt
ABSTRACT
Abd El-Razik
KA, Desouky HM, Ahmed WM, Evaluation of different Brucella
diagnostic techniques on Baladi Does
experimentally infected
with Brucella melitensis, Online Journal
of Veterinary
Research, 10 (2) : 219-234, 2006. Diagnostic methods for
early detection of brucellosis
(Br. melitensis)
were evaluated in does. Ten mature
pregnant Baladi does were used as
controls or
inoculated intraconjunctivally with Br. melitensis biovar
3 between
40 and 45 days of gestation. Blood samples were collected
weekly for immunological
tests. Does with abortions were necropsied
together
with the rest of animals, 3 months later for bacteriological, PCR and
pathological investigations. Results indicated that does inoculated
with Br.
melitensis showed severe respiratory
disorders
and abortion, 3 – 5 weeks post inoculation. Br. melitensis was
isolated from the spleen, and
lymph nodes from all inoculated does. Indirect
Enzyme Linked Immunosorbent Assay (ELISA), Buffered
Acidified Plate Antigen
Test (BAPAT)
and Rose
Bengal Plate Antigen Test (RBPT) showed very early
detection of infection in the serum
(4-7th days post inoculation, DPI). Rivanol
Test (RVT)
showed
relatively early detection (7th DPI) while, Complement
Fixation Test (CFT) and
Tube
Agglutination Test (TAT) showed late detection
of antibody titer (begins 14th
DPI). A significant lymphocyte transformation response among the
different
SBP50 concentrations was noticed in inoculated does. All tissues
specimens
taken from inoculated does yield positive PCR product specific for brucella. The histopathological
examination revealed diffuse suppurative
and
necrotizing placentitis, ulcerative endometritis and presence of large colonies of brucellae, either extra or intracellularly.
It could be concluded that for diagnosis of brucellosis in goats, RBPT
should
be combined with iELISA as screening
tests, while CFT
and /or PCR could be used as a confirmatory test. Detecting colonies of
brucellae (extra-or intra cellular) in the
placental tissue
of aborted does is the most specific pathological lesion.
Key
words: goats – Brucella
melitensis - serology - PCR – pathology
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