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OJVRTM
Online
Journal
of Veterinary
Research©
Volume
11(2):55-61,
2007
Buffalo semen quality,
antioxidants and peroxidation during
chilling and cryopreservation
El-Sisy GA,
El-Nattat WS, El-Sheshtawy RI.
Department
of Animal Reproduction and artificial insemination, National Research
Centre, Dokki, Giza, Egypt
ABSTRACT
El-Sisy GA, El-Nattat
WS, El-Sheshtawy RI, Buffalo semen quality:, antioxidants and peroxidation
during chilling and cryopreservation,
Online J Vet
Res, 11(2):55-61,
2007. Buffalo sperm
quality, antioxidant enzymes (SOD and GSH-Px),
total
antioxidant capacity and lipid peroxidation
during chilling
and cryopreservation were evaluated in 5
Bulls. Sperm
motility declined 87% before processing to 83%, 68% and 37% after
dilution,
cooling and freeze-thawing, respectively. Membrane integrity decreased 90.8% to
83.6%, 72.8% and 51.8%. Normal acrosome
97.80% in raw
semen to 96.4, 77% and 50%, and live sperm from 97.0 % in neat semen to
91.0,
79.4 and 61.4%. After chilling and cryopreservation,
sperm malonyldehyde (MDA) increased from
9.02±1.81 nmol/ml to 12.49±2.19 nmol/ml and
20.69±2.44 nmol/ml, respectively, whereas
SOD decreased
from 5.59±0.04 to 3.98±0.17 and 2.21±0.08 U/ml,
and GSH-Px from 21.68±2.89 to 14.54±2.12
and 10.53±1.51 U/ml. Total antioxidant capacity decreased from
1.96±0.33 to
0.86±0.06
and 0.90±0.18 mM/L.
The results
suggest that buffalo spermatozoa may be affected by
oxidative stress during chilling and
cryopreservation possibly due to a decrease
in antioxidant
enzyme activity.
KEY-WORDS: buffalo – semen – cryopreservation - antioxidants – lipid peroxidation.
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