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OJVRTM
Online Journal of Veterinary Research©
10 (2) 116-121, 2006.
Sequence characterization and phylogenetic analysis of glucose-6-phosphate dehydrogenase gene in Bubalus bubalis
Kumar Ra, Kaur Ra, Kaur Sa, Sethi Pb, Srikala Ra
aDepartment of
Animal Breeding and
Genetics, College of Veterinary Medicine, GADVASU, PAU Campus, Ludhiana, India bDepartment
of
Poultry Science, University of Georgia, 30602,
Athens,USA
Kumar
R,
Kaur R, Kaur
S, Sethi
P, Srikala
R
Sequence
characterization and
phylogenetic analysis of glucose-6-phosphate dehydrogenase gene in
Bubalus bubalis, Online
J Vet Res, 10 (2) 116-121,
2006. Glucose-6-phosphate
dehydrogenase
(G6PD has a crucial role in fatty acid biosynthesis and detoxification
of
organic peroxides. It also indirectly governs the de novo synthesis of
nucleotides by influencing the production of ribose-5-phosphate through
the
hexose monophosphate pathway. In this study, a 683 bp
fragment of buffalo G6PD gene was amplified, sequenced and
characterized. The
deduced amino acid sequence clustered the Bubalus bubalis
G6PD sequence with the other mammalian G6PD proteins into a
monophyletic group.
The multiple sequence alignment of the Bubalus
bubalis G6PD with the mammalian species
clearly revealed
conservation of the substrate and dimer binding sites with the solved
X-ray
crystallographic model structure of Homo sapiens.
Keywords -
Glucose-6-phosphate-dehydrogenase,